Regulatory effect of polyamines and indole on expression of stress adaptation genes in <i> Escherichia coli </i>

Background. Indole and polyamines are involved in the regulation of physiological processes bacteria associated with adaptation to stress, biofilm formation, antibiotic tolerance, bacterial persistence. However, molecular targets mechanisms action these metabolites still poorly understood. In this work, we studied effect indole on expression such genes as: rpoS, relA, spoT, encoding regulators general stress responses starvation; hns stpA, global gene expression; rmf, yqjD, hpf, raiA, rsfS, sra, ettA, ribosome hibernation factors. The aim. To study regulatory effects genes, which responsible for Escherichia coli stress. Materials methods. We used strains E. study. amount was by thin layer chromatography. concentration determined high performance liquid Gene using real-time RT-PCR. Results. addition putrescine, cadaverine spermidine medium stimulated all genes. maximal stimulation observed at stationary phase mostly. Putrescine had most significant effect. At 24 h cultivation, an equimolar conversion exogenous tryptophan into showed. time, two – rmf raiA increased. Conclusions. have shown that upregulate transcriptional level. stimulating is specific batch culture type polyamine. has a positive